Thursday, June 24, 2010

Today we scanned images of DNA samples using AFM. Parts of the process included preparing DNA samples, loading and preparing the AFM and then running the scan. The AFM has two basic modes, oscillating and contact mode. During the contact mode, the cantilever is placed at a specific angle, producing force as it bends. The system works to keep this force and angle constant by raising the cantilever as it moves over a raised surface. In the oscilating mode the image is created when the cantilever acts as a spring and the force can be measured as the surface interacts with the oscillating cantilever and can measure the change in force. At least this is my simplistic view of it. I cannot remember all the details. Olaf gave me an article that explains it well that I will read tonight.

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